Fibre photometry is a powerful technique that measures neural activity in the brains of freely behaving animals. Despite the wide use of this tool, the lack of a structural protocol for its construction and interpretation of data has limited its application. I introduce a detailed protocol of a custom-made fibre photometry setup covering surgery, virus injection, data collection, and analysis. <div><br></div><div>This poster was uploaded for the SGS Research Showcase 2021.<br></div>Choose your favourite poster to win the People's Choice Award at the 2021 SGS Research Showcase: https://auckland.au1.qualtrics.com/jfe/form/SV_9Fw8Omf43pstYUu