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Experimental methods of miRNA target detection.

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posted on 30.10.2014 by Nicole Cloonan, Nicole Cloonan

This figure illustrates the six major methods of experimentally determining miRNA binding sites in mRNAs: (a) genome editing of predicted binding sites; (b) reporter gene assays; (c) gene-expression after miRNA modulation; (d) degradome sequencing; (e) chromatin-linked immuno-precipitation; and (f)

biotin-linked chromatography.

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