Using a ‘one strain-many compounds’ (OSMAC) approach to screen a collection of diverse fungi from Aotearoa New Zealand for antibacterial activity against Escherichia coli
This dataset comprises information on the anti-E. coli activity (measured as zones of inhibition) of 32 fungal isolates grown on seven different media. Fungi were provided by Manaaki Whenua—Landcare Research from the International Collection of Microorganisms from Plants (ICMP) (https://www.landcareresearch.co.nz/tools-and-resources/collections/icmp-culture-collection/). A detailed protocol is available on protocols.io (1). Briefly, we grew each fungal isolate on either Potato Dextrose Agar (PDA), Czapek Solution Agar (CSA), Czapek Yeast Extract Agar (CYA), Malt Extract Agar (MEA), Oatmeal Agar (OA), Rice Extract Agar (REA), and Water Agar (WA) at room temperature until they had grown to cover 20, 50, and 100% of a 90 mm Petri dish (where possible). At each coverage, we used a 6 mm punch biopsy tool (Paramount, Capes Medical, New Zealand) to remove plugs from the growing edge of each fungus. These were placed fungus side down onto Petri dishes of the corresponding media inoculated with a lawn of overnight-grown E. coli ATCC 25922 lux (2) diluted in Mueller Hinton broth to an optical density at 600 nm of 0.01. Plates were incubated at 37 °C overnight, after which we measured the diameter of any zones of inhibition in mm. We also converted the measured diameter into an activity score (0 = 6 mm [the diameter of the fungal plug]; 1 = 7-10 mm; 2 = 11-15 mm; 3 = >15 mm). Data is provided as the zone of inhibition in mm alongside the activity score.
References:
- van de Pas S, Wiles S. Measuring fungal anti-E. coli activity using the zone of inhibition (ZOI) assay. protocols.io. https://www.protocols.io/view/measuring-fungal-anti-e-coli-activity-using-the-zo-ccwwsxfe
- Robertson J, Gizdavic-Nikolaidis M, Swift S. Investigation of Polyaniline and a Functionalised Derivative as Antimicrobial Additives to Create Contamination Resistant Surfaces. Materials 2018;11:436.
