Screening plant pathogenic fungi and their natural products for antibacterial activity.
Raw data from screening a variety of fungal plant pathogens from the International Collection of Microbes from Plants (ICMP) for antibacterial data. Fungi screened were Alternaria radicina (ICMP 5619), Cercospora beticola (ICMP 15907), Dactylonectria macrodidyma (ICMP 16789), D. torresensis (ICMP 20542), Ilyonectria europaea (ICMP 16794) and I. liriodendra (ICMP 16795). Fungi were initially screened for activity against bioluminescent derivatives of Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Mycobacterium abscessus and M. marinum using an adaptation of the method described in 1. Pure compounds obtained from fungal extracts were tested for activity against M. abscessus and M. marinum as described in 2 & 3.
1. Wiles & Grey (2021). Bioluminescence-based 24 well plate assay for screening fungi for activity against Mycobacterium marinum. Protocols.io. DOI: dx.doi.org/10.17504/protocols.io.bvnbn5an.
2. Wiles & Grey (2021). Bioluminescence-based Minimum Inhibitory Concentration (MIC) testing of pure compounds isolated from fungi against Mycobacterium abscessus. Protocols.io. DOI: dx.doi.org/10.17504/protocols.io.bumcnu2w
3. Wiles & Grey (2021). Bioluminescence-based Minimum Inhibitory Concentration (MIC) testing of pure compounds isolated from fungi against Mycobacterium marinum. Protocols.io. DOI: dx.doi.org/10.17504/protocols.io.3x7gprn