In vivo experimental evolution of ICC180, a bioluminescent derivative of the mouse enteropathogen Citrobacter rodentium - part 2
In these experiments, we set up 10 transmission chains in which C. rodentium transmitted naturally from mouse to mouse. Experiments were performed as described in the collection provided on Protocols.io (1). In addition, nalidixic acid was added as a treatment for five of the transmission chains. To do this, mice were given nalidixic acid in their drinking water at a final concentration of 10 ug/mL. This water was changed every 2-3 days, adding fresh nalidixic acid each time.
This dataset contains the processed data for each animal (1-22) in each transmission chain (N1-5, W1-5) - median weight over the experiment (g), weight range (g), change in weight since the start of the infection (%), weight change at the peak of infection (%), bacterial counts (as colony forming units [CFU] per gram of stool) prior to co-mingling, and calculated area under curve values of bacterial shedding (as cfu per gram of stool.time).
1. Read, Patel, & Wiles (2022). Initiating and monitoring natural infection of mice by bioluminescent Citrobacter rodentium. protocols.io. https://www.protocols.io/view/initiating-and-monitoring-natural-infection-of-mic-81wgb6qnnlpk/v1