In vivo experimental evolution of ICC180, a bioluminescent derivative of the mouse enteropathogen Citrobacter rodentium - part 2

<p dir="ltr">In these experiments, we set up 10 transmission chains in which <i>C. rodentium</i> transmitted naturally from mouse to mouse. Experiments were performed as described in the collection provided on Protocols.io (1). In addition, nalidixic acid was added as a treatment for five of the transmission chains. To do this, mice were given nalidixic acid in their drinking water at a final concentration of 10 ug/mL. This water was changed every 2-3 days, adding fresh nalidixic acid each time.</p><p dir="ltr">This dataset contains the processed data for each animal (1-22) in each transmission chain (N1-5, W1-5) - median weight over the experiment (g), weight range (g), change in weight since the start of the infection (%), weight change at the peak of infection (%), bacterial counts (as colony forming units [CFU] per gram of stool) prior to co-mingling, and calculated area under curve values of bacterial shedding (as cfu per gram of stool.time).</p><p dir="ltr">1. Read, Patel, & Wiles (2022). Initiating and monitoring natural infection of mice by bioluminescent Citrobacter rodentium. protocols.io. https://www.protocols.io/view/initiating-and-monitoring-natural-infection-of-mic-81wgb6qnnlpk/v1</p>