2022_10_10_Evolution-exp_year_month_day.csv (196.92 kB)
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In vivo experimental evolution of ICC180, a bioluminescent derivative of the mouse enteropathogen Citrobacter rodentium

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posted on 18.10.2022, 01:32 authored by Siouxsie WilesSiouxsie Wiles, Hannah ReadHannah Read

In these experiments, we set up 10 transmission chains in which C. rodentium transmitted naturally from mouse to mouse. Experiments were performed as described in the collection provided on Protocols.io (1). In addition, nalidixic acid was added as a treatment for five of the transmission chains. To do this, mice were given nalidixic acid in their drinking water at a final concentration of 10 ug/mL. This water was changed every 2-3 days, adding fresh nalidixic acid each time. 


This dataset contains the weights (g), bacterial viability (as colony forming units [CFU] per gram of stool and as relative light units per gram of stool), and bioluminescence from abdomen and rectum (as photons per second).


1. Read, Patel, & Wiles (2022). Initiating and monitoring natural infection of mice by bioluminescent Citrobacter rodentium. protocols.io. https://www.protocols.io/view/initiating-and-monitoring-natural-infection-of-mic-81wgb6qnnlpk/v1

Funding

University of Auckland grant-in-aid (9802-3701152)

Maurice Wilkins Centre for Molecular Biodiscovery grant-in-aid (9431-48516)

History

Publisher

University of Auckland