Antimicrobial activity of the endophytic fungus Neofusicoccum australe
datasetposted on 22.02.2020 by Siouxsie Wiles
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
To perform zone of inhibition (ZOI) testing, potato dextrose agar (PDA) plates were inoculated with a lawn of either antibiotic-sensitive Escherichia coli (ATCC 25922) or resistant clinical isolates (CTX-M-9, CTX-M-14, CTX-M-15, NDM-1) or antibiotic-resistant Klebsiella pneumoniae (ATCC 700603). Similarly, Mueller-Hinton agar plates were inoculated with a lawn of either antibiotic-sensitive Staphylococcus aureus (ATCC 29213) or antibiotic-resistant S. aureus (ATCC 33593). Neofusicoccum australe was grown on PDA plates and fungal plugs removed using a 6 mm punch biopsy tool (Catalogue number: SH241, Amtech Medical, New Zealand). Fungal plugs were placed onto the bacterial lawns, alongside PDA plugs containing no fungus. Plates were incubated inverted at 37°C for 24 h before measuring any zones of inhibition (in mm) produced.