%0 Generic %A Wiles, Siouxsie %D 2020 %T Antimicrobial activity of the endophytic fungus Neofusicoccum australe %U https://auckland.figshare.com/articles/dataset/Antimicrobial_activity_of_the_endophytic_fungus_Neofusicoccum_australe/11888184 %R 10.17608/k6.auckland.11888184.v1 %2 https://auckland.figshare.com/ndownloader/files/21803454 %K zone of inhibition test %K Antibacterial activities %K Escherichia coli %K Staphylococcus aureus %K Klebsiella pneumoniae %K fungus %K Microbiology %X To perform zone of inhibition (ZOI) testing, potato dextrose agar (PDA) plates were inoculated with a lawn of either antibiotic-sensitive Escherichia coli (ATCC 25922) or resistant clinical isolates (CTX-M-9, CTX-M-14, CTX-M-15, NDM-1) or antibiotic-resistant Klebsiella pneumoniae (ATCC 700603). Similarly, Mueller-Hinton agar plates were inoculated with a lawn of either antibiotic-sensitive Staphylococcus aureus (ATCC 29213) or antibiotic-resistant S. aureus (ATCC 33593). Neofusicoccum australe was grown on PDA plates and fungal plugs removed using a 6 mm punch biopsy tool (Catalogue number: SH241, Amtech Medical, New Zealand). Fungal plugs were placed onto the bacterial lawns, alongside PDA plugs containing no fungus. Plates were incubated inverted at 37°C for 24 h before measuring any zones of inhibition (in mm) produced. %I The University of Auckland