%0 Generic
%A Wiles, Siouxsie
%D 2020
%T Antimicrobial activity of the endophytic fungus Neofusicoccum australe
%U https://auckland.figshare.com/articles/dataset/Antimicrobial_activity_of_the_endophytic_fungus_Neofusicoccum_australe/11888184
%R 10.17608/k6.auckland.11888184.v1
%2 https://auckland.figshare.com/ndownloader/files/21803454
%K zone of inhibition test
%K Antibacterial activities
%K Escherichia coli
%K Staphylococcus aureus
%K Klebsiella pneumoniae
%K fungus
%K Microbiology
%X To perform zone of inhibition (ZOI) testing, potato dextrose agar (PDA) plates
were inoculated with a lawn of either antibiotic-sensitive Escherichia coli
(ATCC 25922) or resistant clinical isolates (CTX-M-9, CTX-M-14, CTX-M-15,
NDM-1) or antibiotic-resistant Klebsiella pneumoniae (ATCC 700603).
Similarly, Mueller-Hinton agar plates were inoculated with a lawn of either
antibiotic-sensitive Staphylococcus aureus (ATCC 29213) or
antibiotic-resistant S. aureus (ATCC 33593). Neofusicoccum australe
was grown on PDA plates and fungal plugs removed using a 6 mm punch biopsy tool
(Catalogue number: SH241, Amtech Medical, New Zealand). Fungal plugs were
placed onto the bacterial lawns, alongside PDA plugs containing no fungus. Plates
were incubated inverted at 37°C for 24 h before measuring any zones of
inhibition (in mm) produced.
%I The University of Auckland